G-protein-coupled receptor participates in 20-hydroxyecdysone signaling on the plasma membrane

Cell Communication and Signaling

Research

Jin-Xing Wang¹  Peng-Cheng Liu¹  Wen Liu¹  Yu Wang¹  Xiao-Fan Zhao¹  Mei-Juan Cai¹  Du-Juan Dong¹ 

[1] The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, 250100, Shandong, Jinan, China;

关键词: Steroid hormones;    G-protein-coupled receptors;    Protein phosphorylation;    Calcium influx;    Signal transduction;   

DOI  :  10.1186/1478-811X-12-9

 received in 2013-11-01, accepted in 2014-02-03,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundAnimal steroid hormones are conventionally known to initiate signaling via a genomic pathway by binding to the nuclear receptors. The mechanism by which 20E initiates signaling via a nongenomic pathway is unclear.ResultsWe illustrate that 20E triggered the nongenomic pathway through a plasma membrane G-protein-coupled receptor (named ErGPCR) in the lepidopteran insect Helicoverpa armigera. The transcript of ErGPCR was increased at the larval molting stage and metamorphic molting stage by 20E regulation. Knockdown of ErGPCR via RNA interference in vivo blocked larval–pupal transition and suppressed 20E-induced gene expression. ErGPCR overexpression in the H. armigera epidermal cell line increased the 20E-induced gene expression. Through ErGPCR, 20E modulated Calponin nuclear translocation and phosphorylation, and induced a rapid increase in cytosolic Ca2+ levels. The inhibitors of T-type voltage-gated calcium channels and canonical transient receptor potential calcium channels repressed the 20E-induced Ca2+ increase. Truncation of the N-terminal extracellular region of ErGPCR inhibited its localization on the plasma membrane and 20E-induced gene expression. ErGPCR was not detected to bind with the steroid hormone analog [3H]Pon A.ConclusionThese results suggest that ErGPCR participates in 20E signaling on the plasma membrane.

【 授权许可】

Unknown   
© Cai et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.