The construction and characterization of an effective transpositional system based on IS30

FEBS Letters

Olasz, Ferenc¹  Kiss, János¹  Farkas, Tibor¹ 

[1] Institute for Molecular Genetics, Agricultural Biotechnology Center, Szent-Györgyi A. u. 4., H-2101 Gödöllő, Hungary

关键词: IS30;    Overproduction of IS30 transposase;    In vivo assay;    Transpositional frequency;    Escherichia coli;   

DOI  :  10.1016/0014-5793(96)00626-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We constructed an in vivo system to detect transpositional rearrangements induced by the insertion sequence IS30. The transposase protein expressed from the transposase producer plasmids catalyzed rearrangements on different target sequences presented in trans. High yields, up to 83%, of transpositional frequencies were observed. The frequency of rearrangements correlated with the amount of transposase protein produced and the attractivity of the target sequences. Alteration in the frequency of transposition was observed in the recA ⁻ E. coli strains JM109 and TG2. Remarkable structural and functional analogy was found with site-specific recombination systems.

【 授权许可】

Unknown