Mutations in the carboxy‐terminal part of IS30 transposase affect the formation and dissolution of (IS30)2 dimer

FEBS Letters

Olasz, Ferenc¹  Stalder, Rolf¹  Farkas, Tibor²  Arber, Werner¹ 

[1] Abteilung Mikrobiologie, Biozentrum der Universität Basel, Klingelbergstrasse 70, CH-4056 Basel, Switzerland;Institute for Molecular Genetics, Agricultural Biotechnology Center, Szent-Györgyi Albert utca 4, H-2101 Gödöllő, Hungary

关键词: IS30;    Domain structure of transposase;    Intermediate in transposition;    Site-specific deletion formation;    Insertion sequence;    Escherichia coli;   

DOI  :  10.1016/S0014-5793(97)00947-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The transposase of IS30 catalyses different transpositional rearrangements via the dimer (IS30)₂ intermediate structure. Mutation analysis provides evidence that the C-terminal part of IS30 transposase is required for the formation and dissolution of (IS30)₂ dimer. C-terminal mutants are also defective in transpositional fusion; however, this deficiency can be `suppressed' by addition of the final product of site-specific dimerisation, the core (IS30)₂ intermediate structure. The transposase part studied shows significant homologies in three highly conserved regions to proteins of IS30-related mobile elements.

【 授权许可】

Unknown