Demonstration of the pathogenic effect of point mutated keratin 9 in vivo

FEBS Letters

Kore-eda, Satoshi¹  Kobayashi, Setsu¹  Tanaka, Toshihiro¹ 

[1] Department of Dermatology, Graduate School of Medicine, Kyoto University, Kyoto 606-8507, Japan

关键词: Keratin 9;    Keratin intermediate filament;    Naked DNA transfection;    In vivo assay;    Point mutation;    Immunofluorescence;   

DOI  :  10.1016/S0014-5793(99)00233-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A wild type keratin 9 (K9) cDNA and a point mutated keratin 9 cDNA were injected subcutaneously into mouse skin. The hemagglutinin tag staining of the wild type K9 cDNA injected specimens mainly showed a homogeneous pattern, whereas the point mutated K9 cDNA injected specimens mainly showed a granular pattern in the suprabasal cells. Double staining of K9 and the endogenous keratin revealed the incorporation of de novo synthesized K9 into the keratin network. These results demonstrate that (1) a naked DNA transfection into mouse skin can detect the pathogenic changes of point mutated keratin in vivo and (2) the keratin 9 mutation disrupts the keratin network formation in the suprabasal cells in vivo.

【 授权许可】

Unknown