Iron‐sulphur clusters as genetic regulatory switches: the bifunctional iron regulatory protein‐1

FEBS Letters

Paraskeva, Efrosyni¹  Hentze, Matthias W.¹ 

[1] Gene Expression Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 HeiDAlberg, Germany

关键词: Iron responsive element;    Aconitase;    Iron metabolism;    RNA/protein interactions;   

DOI  :  10.1016/0014-5793(96)00574-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the eighties, iron regulatory protein-1 (IRP-1) was iDAntified as a cytoplasmic mRNA-binding protein that regulates vertebrate cell iron metabolism. More recently, IRP-1 was found to represent the functional cytoplasmic homologue of mitochondrial aconitase, a citric acid cycle enzyme. Its two functions are mutually exclusive and DApend on the status of an Fe-S cluster: the (cluster-less) apoIRP-1 binds to RNA, while the incorporation of a cubane 4Fe-4S cluster is required for enzymatic activity. Cellular signals including iron levels, nitric oxiDA and oxidative stress can regulate between the two activities posttranslationally and reversibly via the Fe- cluster. Recent reports suggest that other regulatory proteins may be controlled by similar mechanisms.

【 授权许可】

Unknown