Combined effects of ATP and its analogs on the membrane permeability in transformed mouse fibroblasts

FEBS Letters

Friedberg, Ilan¹  Arav, Ruth¹ 

[1] Department of Cell Research and Immunology, The George S. Wise Faculty of Life Sciences, Tel Aviv University, 69978 Tel Aviv, Israel

关键词: Extracellular ATP;    Membrane potential;    Membrane permeability;    p[NH]ppA;    5′-adenylyl imidodiphosphate;    p[CH2]ppA;    adenosine 5′-[β;    γ-methylene]triphosphate;    TPP+;    tetraphenylphosphorium;    Δφ;    the electrical potential across the cell membrane;    δΔφ;    difference in Δφ;   

DOI  :  10.1016/0014-5793(96)00472-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Extracellular ATP (0.6 mM) induces a marked decrease in the membrane potential, followed by an increase in cell membrane permeability in transformed mouse fibroblasts. The effects of the ATP analogs, p[CH₂]ppA and p[NH]ppA (0.6 mM), on the membrane potential and permeability are much less pronounced. ATP at 0.05 mM has no effect by itself, but markedly increases the analog-induced membrane potential dissipation and permeability. The data suggest that ATP-induced membrane permeation is composed of two processes: One is common to ATP and its analogs and appears to be a receptormediated process. The second is unique for ATP, effective even at low concentration (0.05 mM), and might be mediated by cell surface enzymes, for which ATP, but not its analogs, serves as a substrate.

【 授权许可】

Unknown