Characterization of purinoceptors present on human liver plasma membranes

FEBS Letters

Keppens, Stefaan¹  De Wulf, Henri¹  Vandekerckhove, Ann¹ 

[1] Afdeling Biochemie, Campus Gasthuisberg, Fakulteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium

关键词: Purinoceptor;    Glycogenolysis;    (Human liver);    ATPαS;    adenosine 5′-[α-thio]triphosphate;    ATPα35S;    adenosine 5′-[α-[35S]thio]triphosphate;    ATPγS;    adenosine 5′-[γ-thio]triphosphate;    pp[CH2]pA;    adenosine 5′-[α;    β-methylene]triphosphate;    p[CH2]ppA;    adenosine 5′-[β;    γ-methylene]triphosphate;    p[NH]ppA;    adenosine 5′-[β;    γ-imido]triphosphate;    Ap3A;    diadenosine triphosphate;    Ap4A;    diadenosine tetraphosphate;   

DOI  :  10.1016/0014-5793(89)80448-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using ATPα³⁵S as radioligand, we have detected the presence of specific purinoceptors on human liver plasma membranes. They are characterized by a K _d value of 0.19 μM and a B _max of 24 pmol/mg membrane protein. These purinoceptors belong to the P_2Y subclass as demonstrated by the high degree of similarity with rat liver purinoceptors, previously shown to be P_2Y[(1986) Biochem. J. 240, 367–371] and known to be involved in the control of liver glycogenolysis.

【 授权许可】

Unknown