Evidence for two closely related isozymes of arylamine N‐acetyltransferase in human liver

FEBS Letters

Lottspeich, Friedrich²  Meyer, Urs A.¹  Grant, Denis M.¹ 

[1] Department of Pharmacology, Biocenter, University of Basel, 4056 Basel, Switzerland;Max-Planck Institut für Biochemie, D-8033 Martinsried, FRG

关键词: Genetic polymorphism;    Arylamine N-acetyltransferase;    Purification;    (Human liver);   

DOI  :  10.1016/0014-5793(89)81193-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Acetyl CoA-dependent arylamine N-acetyltransferase (EC 2.3.1.5) is the target of a genetic polymorphism in the metabolism of drugs and carcinogens. N-Acetyltransferase was purified 1000-fold from cytosol of human liver and its identity was verified by amino acid sequence homology of two of its tryptic peptides with published rabbit and chicken N-acetyltransferase sequences. Enzyme activity correlated with the presence of two proteins, NAT-1 and NAT-2, with indistinguishable molecular masses (31 kDa). NAT-1 and NAT-2 could be separated by anion-exchange chromatography and were functionally distinguished by their different apparent affinities for the acceptor amine sulfamethazine (SMZ). Antibodies raised against NAT-1 were able to recognize both isozymes on Western blots.

【 授权许可】

Unknown