Zfp60, a mouse zinc finger gene expressed transiently during in vitro muscle differentiation

FEBS Letters

Passananti, Claudio¹  Rompato, Giovanni¹  De Gregorio, Laura²  Dragani, Tommaso A.²  Perez, Marie¹  Corbi, Nicoletta¹ 

[1] Istituto Pasteur-Fondazione Cenci Bolognetti, Dipartimento di Biopatologia Umana, Sezione di Biologia Cellulare, Università di Roma La Sapienza, Viale Regina Elena 324, 00161 Rome, Italy;Division of Experimental Oncology, Istituto Nazionale Tumori, Via G. Veneziani 1, 20133 Milan, Italy

关键词: cDNA cloning;    Zinc finger protein;    Myogenic differentiation;    Temporal expression;    DNA binding;    Chromosome mapping;   

DOI  :  10.1016/0014-5793(96)00474-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete cDNA coding sequence of the zinc finger gene Zfp60 is reported. The predicted amino acid sequence of the Zfp60 protein has been found to contain 19 zinc finger motives clustered at the C-terminus. At its N-terminus, Zfp60 shares with other members of the zinc finger gene family two additional conserved amino acid modules named Kruppel Associated Boxes (KRAB). The expression patterns of Zfp60, MyoD and MHC mRNAs have been followed during in vitro myogenic differentiation of C2 cells. We show that the bacterial produced Zfp60 protein binds DNA only in presence of zinc ions. Zfp60 locus has been mapped in chromosome 7, where other Zfp loci are localised.

【 授权许可】

Unknown