Expression of murine novel zinc finger proteins highly homologous to Drosophila ovo gene product in testis

FEBS Letters

Okuda-Ashitaka, Emiko¹  Sato, Eimei²  Ikeda, Sakahiro³  Ito, Seiji¹  Masu, Yasuo¹ 

[1] Department of Medical Chemistry, Kansai Medical University, 10-15 Fumizono, Moriguchi, Osaka 570, Japan;Animal Reproduction Unit, Graduate School of Agriculture, Tohoku University, 1-1 Amamiyamachi, Tutumidori, Aoba-ku, Sendai 981, Japan;Department of Anesthesiology, Kansai Medical University, 10-15 Fumizono, Moriguchi, Osaka 570, Japan

关键词: cDNA cloning;    Zinc finger protein;    ovo;    Homologue;    Spermatogenesis;   

DOI  :  10.1016/S0014-5793(97)01567-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned two isoforms of cDNAs encoding novel zinc finger proteins. One form encodes a 274-amino acid protein containing an acidic amino acid and serine-rich domain and a zinc finger domain which shows high sequence homology to that of Drosophila Ovo protein. The other form encodes a 179-amino acid protein containing only the zinc finger domain. Expression of both proteins possessing an antigenic epitope in COS cells revealed that they are localized in the nucleus. The 1.3-kbp mRNAs are predominantly expressed in testis, and the expression increases from 3 weeks postnatal, implying that these proteins may play important roles in the development of the testes.

【 授权许可】

Unknown