PeerJ | |
Global miRNA expression is temporally correlated with acute kidney injury in mice | |
article | |
Rui Cui1 Jia Xu1 Xiao Chen2 Wenliang Zhu3 | |
[1] Department of Nephrology, The Fourth Affiliated Hospital of Harbin Medical University;Department of Nephrology, Heilongjiang Province Hospital;Institute of Clinical Pharmacology, The Second Affiliated Hospital of Harbin Medical University | |
关键词: Acute kidney injury; MicroRNA; Microarray; Serum creatinine; Temporal expression; | |
DOI : 10.7717/peerj.1729 | |
学科分类:社会科学、人文和艺术(综合) | |
来源: Inra | |
【 摘 要 】
MicroRNAs (miRNAs) are negative regulators of gene expression and protein abundance. Current evidence shows an association of miRNAs with acute kidney injury (AKI) leading to substantially increased morbidity and mortality. Here, we investigated whether miRNAs are inductive regulators responsible for the pathological development of AKI. Microarray analysis was used to detect temporal changes in global miRNA expression within 48 h after AKI in mice. Results indicated that global miRNA expression gradually increased over 24 h from ischemia reperfusion injury after 24 h, and then decreased from 24 h to 48 h. A similar trend was observed for the index of tubulointerstitial injury and the level of serum creatinine, and there was a significant correlation between the level of total miRNA expression and the level of serum creatinine (p < 0.05). This expression-phenotype correlation was validated by quantitative reverse transcription PCR on individual miRNAs, including miR-18a, -134, -182, -210 and -214. Increased global miRNA expression may lead to widespread translational repression and reduced cellular activity. Furthermore, significant inflammatory cytokine release and peritubular capillary loss were observed, suggesting that the initiation of systematic destruction programs was due to AKI. Our findings provide new understanding of the dominant role of miRNAs in promoting the pathological development of AKI.
【 授权许可】
CC BY
【 预 览 】
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