Calnexin : its molecular cloning and expression in the liver of the frog, Rana rugosa

FEBS Letters

Yamamoto, Satoshi¹  Nakamura, Masahisa¹ 

[1] Laboratory for Amphibian Biology, Faculty of Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739, Japan

关键词: Frog;    Calnexin;    cDNA cloning;    Expression in liver cells;   

DOI  :  10.1016/0014-5793(96)00443-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 2.2-kb cDNA clone encoding the endoplasmic reticulum (ER) resident protein, calnexin (CLX), was isolated from the frog Rana rugosa liver cDNA library and sequenced. The sequence encoded 622 amino acids and was 77% similar to mammalian CLXs and 56% to mouse CLX-t. In the phylogenetic tree, mouse CLX-t was clearly diverged from other CLXs including frog. The amino acid sequence of CLX showed regions similar to those in frog calreticulin (CLT), although CLX, but not CLT, contained a histidine-rich region at the NH₂-terminus. CLX gene expression was observed only in the liver among various tissues examined. Additionally, its expression was strong in the liver in 2-month post-metamorphosis frogs, but very weak in adults. The results suggest that the CLX gene is expressed in a tissue- and stage-dependent manner in the frog, R. rugosa, and that CLX is widely distributed in eukaryotic organisms.

【 授权许可】

Unknown