The effect of calnexin deletion on the expression level of binding protein (BiP) under heat stress conditions in Saccharomyces cerevisiae

Cellular & Molecular Biology Letters

Yanyan Ji¹  Huili Zhang¹  Youtao Song¹  Bingjie Hu¹  Akio Kato² 

[1]Department of Life Science, Liaoning University, Shenyang, China$$

[2]Department of Biological Chemistry, Yamaguchi University, Yamaguchi, Japan$$

关键词: Calnexin;    Molecular chaperone;    BiP;    Heat stress;   

DOI  :  10.2478/s11658-008-0026-5

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

In order to investigate the effect of calnexin deletion on the induction of the main ER molecular chaperone BiP, we cultured the wild-type and calnexin-disrupted Saccharomyces cerevisiae strains under normal and stressed conditions. The growth rate of the calnexin-disrupted yeast was almost the same as that of the wild-type yeast under those conditions. However, the induced level of BiP mRNA in the ER was evidently higher in calnexin-disrupted S. cerevisiae than in the wild-type at 37°C, but was almost the same in the two strains under normal conditions. The Western blot analysis results for BiP protein expression in the ER showed a parallel in the mRNA levels in the two strains. It is suggested that under heat stress conditions, the induction of BiP in the ER might recover part of the function of calnexin in calnexin-disrupted yeast, and result in the same growth rate as in wild-type yeast.

【 授权许可】

Unknown