The effect of calnexin deletion on the expression level of PDI in Saccharomyces cerevisiae under heat stress conditions

Cellular & Molecular Biology Letters

Yanyan Ji¹  Huili Zhang¹  Jianwei He²  Youtao Song¹  Akio Kato² 

[1] Department of Life Science, Liaoning University, Shenyang, China$$;Department of Biological Chemistry, Yamaguchi University, Yamaguchi, Japan$$

关键词: Calnexin;    Molecular chaperone;    PDI;    Heat stress;   

DOI  :  10.2478/s11658-007-0033-y

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

We cultured calnexin-disrupted and wild-type Saccharomyces cerevisiae strains under conditions of heat stress. The growth rate of the calnexin-disrupted yeast was almost the same as that of the wild-type yeast under those conditions. However, the induced mRNA level of the molecular chaperone PDI in the ER was clearly higher in calnexin-disrupted S. cerevisiae relative to the wild type at 37°C, despite being almost the same in the two strains under normal conditions. The western blotting analysis for PDI protein expression in the ER yielded results that show a parallel in their mRNA levels in the two strains. We suggest that PDI may interact with calnexin under heat stress conditions, and that the induction of PDI in the ER can recover part of the function of calnexin in calnexin-disrupted yeast, and result in the same growth rate as in wild-type yeast.

【 授权许可】

Unknown