FEBS Letters | |
Cell adhesion and integrin binding to recombinant human fibrillin‐1 | |
Reinhardt, Dieter P.2  Timpl, Rupert1  Sakai, Lynn Y.2  Pfaff, Martin1  | |
[1] Max Planck Institut für Biochemie, D-82152 Martinsried, Germany;Shriners Hospital for Crippled Children, Research Department, Portland, OR 97201, USA | |
关键词: Fibrillin-1; Integrin; RGD; Cell adhesion; | |
DOI : 10.1016/0014-5793(96)00325-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Fibrillin-1 is a major constituent of tissue microfibrils that occur in most connective tissues, either in close association with or independent of elastin. To test possible cell-adhesive functions of this protein, we used recombinant human fibrillin-1 polypeptides produced in a mammalian expression system in cell attachment and solid-phase integrin binding assays. Fibrillin-1 polypeptides containing the single RGD sequence located in the fourth 8-cysteine domain, mediated distinct cell adhesion of a variety of cell lines and bound to purified integrin αVβ3. Integrins αIIbβ3, α5β1, α2β1 and α1β1 did not interact with any of the recombinant fibrillin-1 peptides. Our results indicate a novel role for fibrillin-1 in cellular interactions mediated via an RGD motif that is appropriately exposed for recognition by integrin αVβ3.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020302622ZK.pdf | 424KB | download |