The role of the ninth and tenth type III domains of human fibronectin in cell adhesion

FEBS Letters

Mardon, Helen J.¹  Grant, Kate E.¹ 

[1]Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Headington, Oxford, 0X3 9DU, UK

关键词: Fibronectin;    FIII domain;    RGD;    Cell adhesion;    Cell spreading assay;    Protein expression;    FN;    fibronectins;    ECM;    extracellular matrix;    CCBD;    central cell binding domain;    BHK cells;    baby hamster kidney cells;    kDa;    kilodalton;    FCS;    fetal calf serum;    MEM;    minimal essential medium;    GST;    glutathione S-transferase;   

DOI  :  10.1016/0014-5793(94)80137-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fibronectins (FN) contain sites, in addition to the cell recognition site RGD in the tenth type III domain (FIII10), that are required for adhesive activity. The role of FIII10 and the adjacent FIII9 was analysed in functional cell adhesion assays recombinant FIII domains in which the domain boundaries were strictly conserved. FIII9 had no adhesive activity. FIII10, and FIII9 plus FIII10 had less activity than FN, whereas the activity of FIII9-10 was similar to FN. We conclude that FIII9 acts synergistically with FIII10 in cell adhesion, and that this synergy is dependent upon the structural integrity of the FIII9-10 pair of domains.

【 授权许可】

Unknown