Crystallization and preliminary crystallographic analysis of the signal recognition particle SRPΦ14‐9 fusion protein

FEBS Letters

Kapp, Ulrike¹  Cusack, Stephen¹  Åberg, Anders¹  Doublié, Sylvie¹  Birse, Darcy E.A.¹  Strub, Katharina² 

[1] European Molecular Biology Laboratory (EMBL), Grenoble Outstation, c/o ILL, 156X, 38042 Grenoble Cedex 9, France;Département de Biologie Cellulaire, Université de Genève, Sciences III, CH-1211 Geneva 4, Switzerland

关键词: Signal recognition particle (SRP);    Fusion protein;    Purification;    Crystal;    X-ray diffraction;    Synchrotron;    BICINE;    N;    N-bis[2-hydroxyethyl]glycine hydrochloride;    CAPS;    3-[cyclohexylamino]-1-propanesulfonic acid;    EDTA;    N;    N′-1;    2-ethanediylbis[N-(carboxymethyl]glycine;    EMBL;    European Molecular Biology Laboratory;    ESRF;    European Synchrotron Radiation Facility;    HEPES;    N-[2-hydroxyethyl]piperazine-N′-[2′-ethanesulfonic acid];    IPTG;    isopropyl-β-d-thiogalactopyranoside;    MES;    2-[N-morpholino]ethanesulfonic acid;    MPD;    2-methyl-2;    4-pentanediol;    PAGE;    polyacrylamide gel electrophoresis;    PEG;    polyethylene glycol;    PMSF;    phenylmethylsulfonyl fluoride;    RER;    rough endoplasmic reticulum;    SDS;    sodium dodecyl sulfate;    SRP;    signal recognition particle;    SRP9/14;    signal recognition particle proteins SRP9 and SRP14 heterodimer;    SRPΦ14-9;    SRP9/14 fusion protein;    TRIS;    tris[hydroxymethyl]aminomethane hydrochloride;   

DOI  :  10.1016/0014-5793(96)00315-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The SRPΦ14-9 fusion protein, which can functionally replace the SRP9/14 heterodimer in the mammalian signal recognition particle (SRP), has been crystallized using the vapor diffusion method. Four different crystal forms were grown. SRPΦ14-9 form IV crystals belong to the space group P4₁22/P4₃22 with cell parameters . A complete data set to 2.8 Å resolution with an R _sym on intensities of 7.0% was collected on a single flashfrozen crystal.

【 授权许可】

Unknown