Expression in E. coli and purification of a chimeric p22‐NS3 recombinant antigen of Hepatitis C Virus (HCV)

FEBS Letters

Osborne, S.²  Bonelli, F.²  Rosa, C.²  Calogero, R.¹  Garetto, F.²  Cecconato, E.²  Griva, S.² 

[1] Dipartimento di Genetica, Biologia Generale e Molecolare, Università di Napoli ‘Federico II’, 80134 Napoli, Italy.;R&D Diagnostic Division, SORIN Biomedica S.p.A., 13040 Saluggia (VC), Italy

关键词: Hepatitis C virus;    Antigen;    Fusion protein;    Purification;    Antibody;    HCV;    hepatitis C virus;    anti-HCV;    anti-HCV antibodies;    NS;    non structural;    p22;    nucleocapsid protein;    p22-NS3;    recombinant chimeric antigen;    NANBH;    non-A non-B hepatitis;    Anti-p22;    anti-nucleocapsid antibodies;    Anti-NS3;    anti-NS3 antibodies;    CH6C1N3;    guanidinium chloride;    rec;    baculovirus recombination sequence;    PI;    lambda promoter;   

DOI  :  10.1016/0014-5793(93)80128-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A recombinant antigen (p22-NS3), possessing putative HCV nucleocapsid protein (p22) and non-structural protein 3 (NS3) epitopes, was heavily expressed in E. coli and purified. The p22-NS3 purified recombinant antigen strongly reacts with sera containing human antibodies directed against p22 and NS3 providing a starting point for the design of an HCV single all-encompassing antigen for a blood screening assay.

【 授权许可】

Unknown