Purification of recombinant hepatitis delta antigen expressed in E. coli cells

FEBS Letters

Osborne, S.¹  Bonelli, F.¹  Poisson, F.³  Calogero, R.²  Borla, M.¹  Barbieri, U.¹ 

[1] R&D Diagnostic Division, SORIN Biomedica S.p.A., 13040 Saluggia (VC, Italy;Dipartimento di Genetica, Biologia Generale e Molecolare, Università di Napoli ‘FedericoII’, 80134 Napoli, Italy;Unité de Virologie, Département de Microbiologie Médicale et Moléculaire, URA CNRS 1334, CHU Bretonneau, 2 Boulevard Tonnellé, 37044 Tours Cedex, France

关键词: Hepatitis delta antigen;    Fusion protein;    Purification;    HD;    hepatitis delta;    HDV;    hepatitis delta virus;    HBV;    hepatits B virus;    HDAg;    delta antigen;    recHDAg;    recombinant delta antigen;    HBsAg;    hepatitis B surface antigen;    TFA;    trifluoroacetic acid;    CH6ClN3;    guanidinium chloride;    CH3CN;    acetonitrile;    kDa;    kilodalton;    kb;    kilobase;    anti-HD;    anti-HDV antibodies;    BSA;    bovine serum albumin;    OVA;    ovin serum albumin;   

DOI  :  10.1016/0014-5793(93)80538-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recombinant DNA technology enables the massive production of recombinant hepatitis delta antigen (recHDAg) retaining immunological properties and transport functions. However, purification procedures of the recombinant delta antigen have, to date, not been described in the literature. We present a purification procedure allowing one to obtain highly purified recHDAg from bacterial cells expressing the hepatitis delta antigen.

【 授权许可】

Unknown