DNA affinity to biological membranes is enhanced due to complexation with hydrophobized polycation

FEBS Letters

Yaroslavov, A.A.¹  Kabanov, V.A.¹  Yaroslavova, E.G.¹  Obolsky, O.L.¹  Kabanov, A.V.¹  Sukhishvili, S.A.¹ 

[1] Department of Polymer Sciences, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Leninskie Gory, Moscow 119899, Russian Federation

关键词: DNA-containing complex;    Liposome;    Polyelectrolyte adsorption;    PC;    phosphatidylcholine;    CL;    cardiolipin;    PEA-FITC;    dipalmitoylphosphatidylethanolamine;    N-fluorescein isothiocyanyl;    P2;    4-vinylpyridine/N-ethyl-4-vinylpyridinium bromide copolymer (7/93);    P2;    16′ 4-vinylpyridine/N-ethyl-4-vinylpyridinium bromide/N-cetyl-4-vinylpyridinium bromide copolymer (36/60/4);    D e;    mean hydrodynamic diameter;    EPM;    electrophoretic mobility;    DP;    degree of polymerization;    IPEC;    interpolyelectrolyte complex;   

DOI  :  10.1016/0014-5793(96)00281-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction of negatively charged liquid phosphatidylcholine/cardiolipin liposomes with water-soluble negatively charged DNA/cetylpyridinium bromide and DNA/poly(N-alkyl-4-vinylpyridinium bromide) complexes was studied. It is shown that the DNA/cetylpyridinium bromide complex while interacting with the liposomes is destroyed, so that the cetylpyridinium cation is incorporated into the liposomal membrane and DNA remains in the solution. The DNA/poly-(N-ethyl-4-vinylpyridinium bromide) complex does not interact at all with the liposomes. On the contrary, the complex of DNA with the poly(vinylpyridinium) cation carrying a small amount of N-cetyl groups is adsorbed on the membrane as a whole. The data obtained indicate that complexation of DNA with hydrophobized polycations can be used for enhancing DNA affinity to biological membranes.

【 授权许可】

Unknown