A new application for liposomes in cancer therapy

FEBS Letters

van Muijen, M.²  Haisma, H.J.²  Crommelin, D.J.A.¹  Vingerhoeds, M.H.¹  Storm, G.¹  van de Rijt, R.B.J.¹ 

[1] Department of Pharmaceutics, Faculty of Pharmacy, Utrecht University, PO Box 80.082, 3508 TB Utrecht, The Netherlands;Department of Medical Oncology, Free University Hospital, Amsterdam, The Netherlands

关键词: Monoclonal antibody;    Liposome;    Antibody-directed enzyme prodrug therapy;    ADEPT;    Targeted drug delivery;    ADEPT;    antibody-directed enzyme prodrug therapy;    CHOL;    cholesterol;    DMEM;    Dulbecco's Modified Eagle's Medium;    DTT;    dithiothreitol;    Epi-glu;    epirubicin-glucuronide;    GUS;    β-glucuronidase;    HEPES;    4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid;    MEM;    minimal essential medium;    MPB-PE;    N-[4-(p-mnleimido-phenyl)butyryl] phosphatidyl ethanolamine;    PBS;    phosphate-buffered saline;    PC;    egg l-α-phosphatidylcholine;    PG;    egg-phosphatidylglycerol;    SRB;    sulforhodamine-B;    TCA;    trichloroacetic acid;    TL;    total lipid (phospholipid + cholesterol);   

DOI  :  10.1016/0014-5793(93)80861-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have tested a new type of immunoliposomes which may effectively mediate the targeting of enzymes to be used for site-specific prodrug activation (immuno-enzymosomes). The enzyme β-glucuronidase, capable of activating the prodrug epirubicin-glucuronide (epi-glu), was coupled to the external surface of immunoliposomes directed towards ovarian cancer cells. A significant increase in cytotoxicity of the prodrug epi-glu was shown when the in vitro cultured cancer cells were pretreated with these immuno-enzymosomes.

【 授权许可】

Unknown