Monoclonal antibody to human endothelial cell surface internalization and liposome delivery in cell culture

FEBS Letters

Rudin, A.V.¹  Domogatsky, S.P.¹  Nikolayeva, M.N.¹  Trubetskaya, O.V.¹  Trubetskoy, V.S.¹  Popov, N.V.¹  Torchilin, V.P.¹  Danilov, S.M.¹  Klibanov, A.L.¹ 

[1] USSR Cardiology Research Center, Academy of Medical Sciences, 121552 Moscow, USSR

关键词: Monoclonal antibody;    Avidin-biotin;    Liposome;    Targeting;    Internalization;    (Endiothelial cell);    mAb;    monoclonal antibody;    EC;    endothelial cell(s);    HRPO;    horseradish peroxidase;    DPPC;    dipalmitoylphosphatidylcholine;    DPPE;    dipalmitoylphosphatidylethanolamine;    BAC-DPPE;    biotinyl amidocaproyl-DPPE;    HPA-DPPE;    p-hydroxyphenylpropionylamido-DPPE;    ILC;    immunoliposome complex(es);   

DOI  :  10.1016/0014-5793(88)80601-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A monoclonal antibody (mAb), E25, is described that binds to the surface of cultured human endothelial cells. Upon binding E25 is rapidly internalized and digested intracellularly. Selective liposome targeting to the surface of the cells is performed using a biotinylated E25 antibody and an avidin-biotin system. Up to 30% of the cell-adherent liposomal lipid is internalized.

【 授权许可】

Unknown