Long circulating, cationic liposomes containing amino‐PEG‐phosphatidylethanolamine

FEBS Letters

Brandeis, Ester¹  Woodle, Martin C.¹  Zalipsky, Samuel¹  Newman, Mary S.¹ 

[1] Liposome Technology Inc., 960 Hamilton Ct., Menlo Park, CA 94025, USA

关键词: Liposome;    Polyethylene glycol;    Targeting;    DNA;    PEG;    polyethylene glycol;    mPEG;    methoxy-PEG;    PE;    phosphatidylethanolamine;    mPEG-PE;    conjugate of mPEG and PE;    aminoPEG-PE;    conjugate of aminoPEG and PE;    TEA;    triethylamine;    SC;    succinimidyl carbonate;    TLC;    thin layer chromatography;    Boc;    tert-butyloxycarbonyl;    PC;    phosphatidylcholine;    DF;    desferoxamine mesylate;    DSPE;    1;    2-disteoroyl-sn-glycero-3-phosphoethanolamine;   

DOI  :  10.1016/0014-5793(94)01013-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ligand attachment to polyethylene glycol (PEG) grafted, long circulating liposomes at the polymer terminus is of interest for targeting but the effect of positively charged groups is unknown. Amino-polyethylene glycol-phosphatidylethanolamine (AminoPEG-PE), prepared in four steps from α-amino-ω-hydroxy-PEG, was tested for influence on liposome interactions in vivo: blood circulation and biodistribution. Despite surface amines on each liposome conferring cationic behavior, in vivo properties are comparable to those obtained with methoxy-PEG-PE. The consequences are profound for targeting and possibly systemic delivery of cationic lipidic-polynucleotide complexes.

【 授权许可】

Unknown