Peroxynitrite formed by simultaneous generation of nitric oxide and superoxide selectively inhibits bovine aortic prostacyclin synthase

FEBS Letters

Ullrich, V.¹  Zou, M.-H.¹ 

[1] Faculty of Biology, University of Konstanz, D 78434 Konstanz, Germany

关键词: Peroxynitrite;    Hypochlorite;    Prostacyclin synthase;    Ischemia-reperfusion;    Irreversible inhibition;    Circulation;   

DOI  :  10.1016/0014-5793(96)00160-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of various oxidants on bovine aortic prostacyclin synthase was tested with ¹⁴C-labelled prostaglandia endoperoxide as substrate. No sensitivity against hydrogen peroxide, superoxide or hydroxyl radicals was observed but hypochlorite inhibited with an IC₅₀ value of 7 μM. Among the reactive nitrogen species nitric oxide and nitrogen dioxide radicals were ineffective, but peroxynitrite irreversibly blocked prostacyclin biosynthesis with an IC₅₀ value of 50 nM. Peroxynitrite acted within seconds whereas hypochlorite required up to 30 min for completion. Simultaneous generation of nitric oxide and superoxide also caused inhibition which suggested that under pathological conditions like ischemia-reperfusion not only the vasodilatory effects of nitric oxide but also those of prostacyclin could be eliminated.

【 授权许可】

Unknown