Identification of RII‐binding proteins in the mollusc Mytilus galloprovincialis

FEBS Letters

Fernández, Montserrat¹  Ramos-Martínez, J.Ignacio¹  Antonio Villamarín, J¹  Cao, Jesús¹ 

[1] Departamento de Bioquimica e Bioloxia Molecular, Facultade de Veterinaria, Universidade de Santiago de Compostela, Campus de Lugo, E-27002 Lugo, Spain

关键词: cAMP-dependent protein kinase;    Regulatory subunit;    R-binding protein;    Kinase anchor protein;    Mollusc;    cAPK;    cAMP-dependent protein kinase (EC 2.7.1.37);    C and R;    Catalytic and regulatory subunits of cAPK;    RII;    type II regulatory subunit;    PheMeSO2F;    phenylmethylsulfonyl fluoride;   

DOI  :  10.1016/0014-5793(96)00158-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Several proteins with M _r 70 kDa from various tissues of the sea mussel Mytilus galloprovincialis were specifically recognized in vitro by the regulatory subunit (type RIIα) of cAMP-dependent protein kinase (cAPK) from porcine heart. However, none of these proteins interacted with the regulatory subunit of cAPK from the mollusc itself. The results suggest that, unlike mammalian RII, regulatory subunit from mussel lacks the specific residues responsible for interaction with R-binding proteins. Consequently, the identified molluscan RIIα-binding proteins should play a distinct role from cAPK anchoring.

【 授权许可】

Unknown