Expression cloning of a cDNA encoding the type II regulatory subunit of the cAMP‐dependent protein kinase

FEBS Letters

Schwarz, Manuela¹  Jans, David A.¹  Hemmings, Brian A.¹  Rao Adavani, S.¹ 

[1] Friedrich M iescher-Institut, PO Box 2543, CH-4002 Basel, Switzerland

关键词: cyclic AMP dependence;    Protein kinase;    Regulatory subunit;    Expression cloning;    (LLC-PK1 cell);   

DOI  :  10.1016/0014-5793(86)81114-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report here the isolation and sequence of a cDNA for the type II regulatory subunit of the cAMP-dependent protein kinase (cAMP-PK) from a λgt-11 cDNA library derived from a porcine epithelial cell line (LLC-PK₁). The cDNA was detected by immunological screening using an affinity purified polyclonal antibody for bovine R_II. DNA sequence analysis of the 467 bp EcoRI insert confirmed the identity of the clone, because the deduced amino acid sequence corresponded to the published sequence for the bovine R_Π protein. Northern analysis of total RNA from the LLC-PK₁ cells indicated a single mRNA species of about 6.0 kb, probably derived from a single copy gene.

【 授权许可】

Unknown