FEBS Letters | |
Mitochondrial free calcium transients during excitation‐contraction coupling in rabbit cardiac myocytes | |
Lemasters, John J1  Harper, Ian S1  Ohata, Hisayuki1  Chacon, Enrique1  Herman, Brian1  Trollinger, Donna R1  | |
[1] Laboratories for Cell Biology, Department of Cell Biology & Anatomy and Curriculum in Toxicology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7090, USA | |
关键词: Calcium; Confocal microscopy; Excitation-contraction coupling; Mitochondria; Myocyte; | |
DOI : 10.1016/0014-5793(96)00138-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Mitochondrial free Ca2+ may regulate mitochondrial ATP production during cardiac exercise. Here, using laser scanning confocal microscopy of adult rabbit cardiac myocytes co-loaded with Fluo-3 to measure free Ca2+ and tetramethylrhodamine methylester to identify mitochondria, we measured cytosolic and mitochondrial Ca2+ transients during the contractile cycle. In resting cells, cytosolic and mitochondrial Fluo-3 signals were similar. During electrical pacing, transients of Fluo-3 fluorescence occurred in both the cytosolic and mitochondrial compartments. Both the mitochondrial and the cytosolic transients were potentiated by isoproterenol. These experiments show directly that mitochondrial free Ca2+ rises and falls during excitation-contraction coupling in cardiac myocytes and that changes of mitochondrial Ca2+ are kinetically component to regulate mitochondrial metabolism on a beat-to-beat basis.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020302435ZK.pdf | 730KB | download |