A preferential pole for exocytosis in cultured chromaffin cells revealed by confocal microscopy

FEBS Letters

Garcı́a, Antonio G.¹  Cuchillo-Ibáñez, Inmaculada¹  Albillos, Almudena¹  Michelena, Pedro¹ 

[1] Departamento de Farmacologı́a, Facultad de Medicina, Universidad Autónoma de Madrid, Arzobispo Morcillo 4, 28029 Madrid, Spain

关键词: Exocytosis;    Chromaffin;    Confocal microscopy;    Polarity;    Calcium;   

DOI  :  10.1016/S0014-5793(99)01115-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Histological studies suggest that adrenal medulla chromaffin cells in situ are polarised, but functional evidence is lacking. We present here the first demonstration for polarisation of exocytosis in isolated, spherical, bovine chromaffin cells. Cells were stimulated with 70 mM K⁺ to cause a marked enhancement of catecholamine release, monitored amperometrically. FM1-43 and dopamine β-hydroxylase antibodies provided fluorescence confocal pictures that were 2–3-fold more intense in the bottom of the cells, as compared to equatorial or apex planes. This suggests that the solid phase to which the cell attaches serves as a ‘trophic’ signal for the polarisation of its secretory apparatus.

【 授权许可】

Unknown