Carbohydrate induced modulation of cell membrane: II. Spin label study of fluidity changes in peripheral blood lymphocyte membrane

FEBS Letters

Kayestha, Rajeev¹  Hajela, Krishnan¹ 

[1] School of Life Sciences, Devi Ahilya Vishwavidyalaya, Vigyan Bhawan, Khandwa Road Campus, Indore-452001 (M.P.), India

关键词: Lymphocyte membrane;    Selectin;    Fluidity;    EPR;    5-DS;    Glc NAc;    N-acetylglucosamine 5-doxyl-stearic acid;    16-DS;    16-doxyl stearic acid;    MECP;    3-(2-maleimidoethyl carbomyl) proxyl;    TEMPO;    2;    2;    6;    6 tetramethyl-1 piperidine N-oxyl;    PBS;    0.01 M phosphate buffer pH 7.4 containing 0.15 M NaCl and 0.01 M CaCl2;    GPBL;    goat peripheral blood lymphocyte;   

DOI  :  10.1016/0014-5793(96)00036-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

This paper reports for the first time, that binding of various mono-, di-, and trisaccharides to membrane lectins reduces the rotational motion of membrane proteins and lipids indicating a decrease in membrane fluidity as studied by EPR spectroscopy using spin probes. Interaction of polysaccharides with lymphocyte resulted in an extensive decrease in membrane fluidity making the membrane almost rigid. The decrease in fluidity was dose-dependent, dependent on the multivalency of the ligand used, and was sensitive to presence of EDTA and sodium azide. Binding of two different carbohydrate ligands on their respective surface lectins has a synergistic effect on the decrease in membrane fluidity.

【 授权许可】

Unknown