Efficient enzymatic synthesis of the sialyl‐LewisX tetrasaccharide

FEBS Letters

Schultz, Jody²  van den Eijnden, Dirk H.¹  de Vries, Theodora¹  O'Neill, Roger² 

[1] Department of Medical Chemistry, Vrije Universiteit, Van der Boechorststraat 7, 1081 BT Amsterdam, The Netherlands;GlycoGen Inc., South San Francisco, CA 94080, USA

关键词: Sialyl-Lex;    αl→3-Fucosyltransferase;    α2→3-Sialyltransferase;    Selectin;    α1-AGP;    α1-acid glycoprotein;    α3-ST;    α2→3-sialyltransferase;    CMP-NeuAc:Ga1β1→4G1cNAc→R;    α2→3-sialyltransferase;    α3-FT;    α1→3-fucosyltransferase;    GDP-Fuc:Ga1β1→ 4GlcNAc→R;    α1→3-fucosyltransferase;    sialyl-LeX;    sialyl-Lewisx;   

DOI  :  10.1016/0014-5793(93)80881-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sialyl-Lewis^x (NeuAcα2→3Galβ1→4[Fucαl→3]GlcNAc] has been identified as a ligand for E-selectin, P-selectin and recently also for L-selectin. We have synthesized the sialyl-Lewis^x tetrasaccharide by total enzymatic synthesis from N-acetyllactosamine using a placental α2→3-sialyltransferase specific for type-2 chain acceptors, followed by a cloned human α1→3-fucosyltransferase (FucTV, the ‘plasma-type’ enzyme). This procedure resulted in the tetrasaccharide in a 61% overall yield.

【 授权许可】

Unknown