Triple selectin knockout (ELP-/-) mice fail to develop OVA-induced acute asthma phenotype

Journal of Inflammation

Research

Ena Ray Banerjee¹ 

[1] Division of Hematology, Department of Medicine, University of Washington, 1959 NE Pacific Street, 98195, Seattle, WA, USA;Immunology and Regenerative Medicine Research Laboratory, Dept of Zoology, University of Calcutta, 35 Ballygunge Circular Road, 700019, Kolkata, India;

关键词: Selectin;    asthma;    inflammation;   

DOI  :  10.1186/1476-9255-8-19

 received in 2010-11-30, accepted in 2011-08-11,  发布年份 2011

来源: Springer

【 摘 要 】

ObjectiveThe recruitment of leukocytes from circulation to sites of inflammation requires several families of adhesion molecules among which are selectins expressed on a variety of cells. In addition, they have also been shown to play key roles in the activation of cells in inflammation.MethodsTo explore the collective role of E-, L-, and P- selectins in OVA-induced Th2 mediated response in acute asthma pathophysiology, ELP-/- mice were used and compared with age-matched wildtype (WT).ResultsAsthma phenotype was assessed by measuring pulmonary function, inflammation and OVA-specific serum IgE, which were completely abrogated in ELP-/- mice. Adoptive transfer of sensitized L selectin+CD4+ T cells into naïve ELP-/- mice which post-OVA challenge, developed asthma, suggesting that L-selectin may be critically involved in the onset of Th2 response in asthma. Tissue resident ELP-deficient cells were otherwise functionally competent as proved by normal proliferative response. Conclusions: Comparative studies between ELP-/- and WT mice uncovered functional roles of these three integrins in inflammatory response in allergic asthma. All three selectins seem to impede inflammatory migration while only L-selectin also possibly regulates activation of specific T cell subsets in lung and airways.

【 授权许可】

CC BY   
© Banerjee; licensee BioMed Central Ltd. 2011