Opposite regulation of bilirubin and 4‐nitrophenol UDP‐glucuronosyltransferase mRNA levels by 3,3′,5 triiodo‐l‐thyronine in rat liver

FEBS Letters

Mounié, Jacques¹  Magdalou, Jacques²  Artur, Yves¹  Goudonnet, Hervé¹  Masmoudi, Taoufik¹  Planells, Richard³ 

[1] Formation de Biochimie Pharmacologique, UFR de Pharmacie, 7 Bv. Jeanne d'Arc, Dijon, France;Centre du médicament, URA CNRS 597, 30 rue Lionnois, 54000 Nancy, France;Laboratoire de Biochimie Médicale, INSERM U38, Faculté de médecine, 13385 Marseille, France

关键词: UDP-glucuronosyltransferase;    Liver (rat);    Gene expression;    3;    3′;    5 TriiodO-l-thyronine;    Bilirubin and 4-nitrophenol glucuronidation;    UGT;    UDP-glucuronosyltransferase;    L-T3;    3;    3′;    5-triiodo-l-thyronine;    RT-PCR;    reverse transcription-polymerase chain reaction;    3-MC;    3-methylcholanthrene;   

DOI  :  10.1016/0014-5793(95)01507-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of 3,3′,5 triiodo-l-thyronine (L-T3) on the constitutive levels of hepatic mRNA encoding two UDP-glucuronosyltransferase (UGT) isoforms implicated in the glucuronidation of planar phenolic substrates (UGTI^∗06) and bilirubin (UGT1^∗0) were investigated in rat liver. The amount of UGT mRNA was quantitated by reverse transcription and amplification methods (RT-PCR). Treatment with L-T3 significantly increased UGT1^∗06 and decreased UGT1^∗0 mRNA levels by 41% and 54%, respectively. The opposite situation was observed in thyroidectomised animals. A good relationship observed between UGT activity toward 4-nitrophenol and bilirubin and mRNA levels emphasizes the key role played by the thyroid hormone L-T3 on UGT expression.

【 授权许可】

Unknown