In vitro Glucuronidation of Estradiol-17β by Microsomes Prepared using Liver Biopsy Specimens from Dairy Cows

Journal of Veterinary Medical Science

Hiroya KADOKAWA¹  Hiroshi YOKOTA²  Junya MATSUMOTO² 

[1] Department of Veterinary Medicine, Faculty of Agriculture, Yamaguchi University;School of Veterinary Medicine, Rakuno Gakuen University

关键词: Estrogen metabolism;    Estrus;    UDP-glucuronosyltransferase;   

DOI  :  10.1292/jvms.69.557

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(12)Increased hepatic metabolism of estradiol may cause weakened estrous behavior in lactating dairy cows, but this hypothesis must be examined further, especially through diachronic study of the hepatic estradiol-17β glucuronidation activity of uridine diphosphate (UDP)-glucuronosyltransferases. Therefore, in order to develop a new tool for this purpose, we attempted to conduct biopsy of the livers of dairy cows with the aid of ultrasonography and to measure the UDP-glucuronosyltransferase activities of microsomes of the specimens using in vitro glucuronidation followed by HPLC analysis. We were able to measure the activities of the microsomes prepared from the liver biopsy, and the results seemed reliable. Therefore, this method may become a new tool in clinical studies to detect estradiol-17β glucuronidation activity.

【 授权许可】

Unknown