Expression of a human liver cDNA encoding a UDP‐glucuronosyltransferase catalysing the glucuronidation of hyodeoxycholic acid in cell culture

FEBS Letters

Jackson, M.R.¹  Fournel-Gigleux, S.¹  Burchell, B.¹  Wooster, R.¹ 

[1] Department of Biochemistry, Medical Sciences Institute, The University, Dundee DD1 4HN, Scotland

关键词: Glucuronosyltransferase;    Bile acid;    Expression;    Cloning;    Substrate specificity;    (COS 7-cell);    UDPGT;    UDP-glucuronosyltransferase;    HLUG25;    human liver UDPGT cDNA;    SV40;    Simian Virus early gene promoter;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(89)80111-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA encoding a human liver UDPGT (HLUG 25) transcribed and translated in vitro showed that the encoded protein was synthesized as a precursor and was cleaved and glycosylated when dog pancreatic microsomes were present during translation. The UDPGT cDNA was transiently expressed in mammalian cell culture (COS-7 cells) resulting in the biosynthesis of a polypeptide of 52 kDa. This expressed UDPGT glycoprotein catalysed the glucuronidation of hyodeoxycholic acid forming an ether glucuronide. These results suggest that this UDPGT isoenzyme may be responsible for the glucuronidation of 6α-hydroxy bile acids in human liver.

【 授权许可】

Unknown