Intercellular Ca2+ waves sustain coordinate insulin secretion in pig islets of Langerhans

FEBS Letters

Pontiroli, A.E.²  Grohovaz, F.³  Pozza, G.²  Berra, C.²  Socci, C.¹  Bertuzzi, F.²  Zacchetti, D.³ 

[1] Surgical Department, San Raffaele Scientific Inst., via Olgettina 58, 20132 Milano, Italy;Medicine Department, DIBIT, San Raffaele Scientific Inst., via Olgettina 58, 20132 Milano, Italy;CNR Cellular and Molecular Pharmacology Ctr and ‘B. Ceccarelli’ Ctr, DIBIT, San Raffaele Scientific Inst., via Olgettina 58, 20132 Milano, Italy

关键词: Islets of Langerhans;    Fura-2;    Ca2+ oscillation;    Ca2+ wave;    Insulin secretion;   

DOI  :  10.1016/0014-5793(95)01422-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Insulin release was investigated in parallel with changes in cytosolic calcium concentration, [Ca²⁺]_i, in pig islets stimulated by glucose. After two days in culture, glucose stimulation failed to induce insulin release, and caused limited [Ca²⁺]_i changes in few cells. After ten days, insulin response was partially restored and [Ca²⁺]_i recordings revealed a slow oscillatory activity of the whole islet. Slow oscillations appeared to be due to the average [Ca²⁺]_i variations resulting from the spreading of waves throughout the islet. These waves demonstrate the reestablishment of functional cell coupling, which appears to play a critical role in insulin release.

【 授权许可】

Unknown