Inositol triphosphate produces different patterns of cytoplasmic Ca2+ spiking depending on its concentration

FEBS Letters

Petersen, Ole H.²  Potter, Barry V.L.¹  Toescu, Emil C.²  Petersen, Carl C.H.² 

[1] School of Pharmacy and Pharmacology, University of Bath, Bath, BA2 7AY, UK;MRC Secretory Control Research Group, The Physiological Laboratory, University of Liverpool, P.O. Box 147, Liverpool, L69 38X, UK

关键词: Ca2+ spike;    Ca2+ wave;    Inositol trisphosphate;    Inositol trisphosphorothioate;   

DOI  :  10.1016/0014-5793(91)81181-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In single mouse pancreatic acinar cells the effects of intracellular infusion of inositol 1,4,5-trisphosphate (InsP₃) or the non-metabolizable InsP₃ analogue inositol 1,4,5-triphosphorothioate (InsPS₃) have been investigated using a wide range of concentrations. Different types of cytosolic Ca²⁺ fluctuation patterns (monitored as Ca²⁺-dependent Cl⁻ current in patch-clamp whole-cell recording experiments) could be generated by InsP₃ or InsPS₃, dependent on concentration, resembling those previously shown to be evoked by varying degrees or receptor activation in these cells. Low InsPS₃ concentrations evoked repetitive local Ca²⁺ spikes whereas at relatively high concentrations repetitive Ca²⁺ waves were produced. In the presence of intracellular citrate a much lower messenger level was sufficient to generate waves. The InsP₃ concentration determines whether the cytosolic Ca²⁺ signals are local or global.

【 授权许可】

Unknown