Schizosaccharomyces pombe possesses an unusual and a conventional hexokinase: biochemical and molecular characterization of both hexokinases

FEBS Letters

Gancedo, Carlos¹  Petit, Thomas¹  Blázquez, Miguel A.¹ 

[1]Instituto de Investigaciones Biomédicas del C.S.I.C, Unidad de Bioquímica y Genética de Levaduras, E-28029 Madrid, Spain

关键词: Hexokinase;    Sugar phosphorylation;    Fructose;    Glucose;    Schizosaccharomyces pombe;   

DOI  :  10.1016/0014-5793(95)01451-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two hexokinases were characterized in Schizosaccharomyces pombe: hexokinase 1, with a low phosphorylation coefficient on glucose (Km 8.5 mM) and hexokinase 2, a kinetically conventional hexokinase. Genes hxk1 ⁺ and hxk2 ⁺ encoding these enzymes were cloned and sequenced. Disruption of hxk1 ⁺ had no effect on growth but disruption of hxk2 ⁺ doubled the generation time in glucose. Spores carrying the double disruption hxk1 ⁺ hxk2 ⁺ did not grow on glucose or fructose after one week. Expression of hxk1 ⁺ increased strongly during growth in fructose or glycerol. Expression of hxk2 ⁺ was highest during growth in glycerol. A NADP-dependent glucose dehydrogenase was detected, but not a glucokinase.

【 授权许可】

Unknown