Isolation of an ion channel gene from Arabidopsis thaliana using the H5 signature sequence from voltage‐dependent K+ channels

FEBS Letters

Ketchum, Karen A.¹  Slayman, Carolyn W.¹ 

[1] Department of Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06510, USA

关键词: K+ channel;    Inward-rectifier;    Plant;    Arabidopsis;    cDNA clone;    Xenopus oocyte expression;   

DOI  :  10.1016/0014-5793(95)01417-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A degenerate oligonucleotide corresponding to the K⁺ channel signature sequence (TMTTVGYGD) was used to isolate the genomic and cDNA forms of a new channel gene, AKT3, from Arabidopsis thaliana. The deduced protein sequence has a predicted membrane topography similar to Shaker-like K⁺ channels. Three distinct modules comprise the carboxyl-terminal half: a nucleotide-binding motif, an ankyrin repeat domain, and a polyglutamate track. Xenopus oocytes injected with cRNA exhibited an inward-rectifying K⁺ current, demonstrating that the AKT3 polypeptide is a functional transport protein. Two other Arabidopsis K⁺ transporters (AKT1 and KAT1) share 60% homology with AKT3; together these proteins constitute a family of plant inward-rectifying K⁺ channels.

【 授权许可】

Unknown