A novel partner for the GTP‐bound forms of rho and rac

FEBS Letters

Furuyashiki, Tomoyuki¹  Morii, Narito¹  Watanabe, Go¹  Ishizaki, Toshimasa¹  Reid, Tim¹  Narumiya, Shuh¹  Madaule, Pascal¹ 

[1] Second Department of Pharmacology, Faculty of Medicine, Kyoto University, Yoshida, Konoe, Sakyo-ku, Kyoto 606, Japan

关键词: Signal transduction;    Small GTP-binding protein;    Two hybrid system;    Coiled-coil;    Zinc finger;    Pleckstrin homology;    p21;    ras-related GTP-binding protein;    PH;    pleckstrin homology;    aa;    amino acid residue;   

DOI  :  10.1016/0014-5793(95)01351-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using the yeast two hybrid system and overlay assays we identified a putative rho/rac effector, citron, which interacts with the GTP-bound forms of rho and rac1, but not with cdc42. Extensive homologies to known proteins were not observed. This 183 kDa protein contains a C₆H₂ zinc finger, a PH domain, and a long coiled-coil forming region including 4 leucine zippers and the rho/rac binding site. We recently identified three others putative rho effectors characterized by a common rho binding motif. Citron does not share this motif and displays a distinctive protein organization, thus defining a separate class of rho partners.

【 授权许可】

Unknown