Quantification of DNA damage and repair in amino acid auxotrophs and UV‐sensitive mutants of Aspergillus nidulans using an ELISA

FEBS Letters

McCullough, W.¹  Barnett, Y.A.²  Donnelly, E.¹ 

[1] School of Applied Biological and Chemical Sciences, University of Ulster at Jordanstown, Jordanstown, Shore Road, Newtownabbey, Co. Antrim BT37 0QB Northern Ireland, UK;School of Biomedical Sciences, University of Ulster at Coleraine, Coleraine, Co. Londonderry BT52 1SA Northern Ireland, UK

关键词: DNA repair;    Single-strand break assay;    ELISA;    Aspergillus nidulans;   

DOI  :  10.1016/0014-5793(95)01316-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An ELISA used to investigate DNA repair in mammalian cells has been adapted to investigate mutagen-induced DNA damage and repair in protoplasts of Aspergillus nidulans. The assay shows a reduced rate of repair of DNA damage in methionine and arginine auxotrophs (methG and argB), which were shown previously to be hypersensitive to UV radiation and chemical mutagens. The assay also showed a considerably reduced ability to repair mutagen-induced damage in the uv-sensitive mutants uvsB and uvsH. The increased sensitivity of amino acids auxotrophs to mutagens is, therefore, correlated with a reduced capacity to repair mutagen-induced DNA damage.

【 授权许可】

Unknown