Poly(U) binding activity of hepatitis C virus NS3 protein, a putative RNA helicase

FEBS Letters

Kohara, Michinori¹  Kanai, Akio¹  Tanabe, Kyoko¹ 

[1] Department of Microbiology, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), Honkomagome, Bunkyo-ku, Tokyo 113, Japan

关键词: Poly(U);    RNA binding;    Helicase;    Hepatitis C virus;    NS3 protein;   

DOI  :  10.1016/0014-5793(95)01283-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A non-structural protein of the hepatitis C virus (HCV), NS3, contains amino acid sequence motifs characteristic of serine-proteinases and RNA helicases. RNA binding activity of the NS3 protein with an apparent dissociation constant of 2 × 10⁻⁷ M was detected using a poly(U)-Sepharose resin. Competitive RNA binding analysis suggested that the NS3 protein binds preferentially to the poly(U) sequence, which is located at the 3′ end of HCV RNA. Mutational analysis of NS3 protein revealed the possibility that both the RNA helicase region and the serine-proteinase region were necessary for full RNA binding activity.

【 授权许可】

Unknown