Zinc is required for structural stability of the C‐terminus of archaeal translation initiation factor aIF2β

FEBS Letters

Gehring, Kalle¹  Gutiérrez, Pablo¹  Coillet-Matillon, Stéphane¹  Arrowsmith, Cheryl² 

[1] McGill University, Department of Biochemistry, McIntyre Medical Science Building, 3655 Promenade Sir William Osler, Montréal, QC, Canada H3G 1Y6;Deparment of Medical Biophysics, University of Toronto, Toronto, ON, Canada M5G 2M9

关键词: aIF2β;    Translation initiation;    Zinc finger;    RNA binding;    Secondary structure;    Nuclear magnetic resonance;   

DOI  :  10.1016/S0014-5793(02)02610-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

aIF2β is an archaeal homolog of eukaryotic translation factor eIF2β necessary for translation initiation and involved in recognition of the initiation codon. In the present study, we demonstrate for the first time zinc binding to the C₂–C₂ zinc finger at the C-terminus of aIF2β. Nuclear magnetic resonance backbone assignments were also determined and the secondary structural elements identified.

【 授权许可】

Unknown