Mono‐, di‐ and trimeric binding of a bis‐netropsin to DNA

FEBS Letters

Zasedatelev, A.S.¹  Nikitin, A.M.¹  Gursky, G.V.¹  Borodulin, V.B.³  Grokhovsky, S.L.¹  Shafer, R.H.²  Zhuze, A.L.¹  Salmanova, D.V.¹ 

[1] Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 117984 Moscow, Russian Federation;Department of Pharmaceutical Chemistry, School of Pharmacy, University of California, San Francisco, CA 94143-0446, USA;University of Oslo Center for Medical Studies, Oslo, Norway

关键词: DNA/drug interaction;    Minor groove binding;    Side-by-side binding;    Circular dichroism;   

DOI  :  10.1016/0014-5793(95)01230-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

An unusual 3:1 stoichiometry for complex formation between an elongated bis-netropsin compound and its binding site on DNA has been observed. Circular dichroism measurements distinguish two types of complexes formed between this bisnetropsin and poly[d(A-T)] · poly[d(A-T)]. The first type is characterized by a 1:1 saturating ratio of bound molecules per ten base pairs. Formation of the second type results from the cooperative binding of two additional bis-netropsin molecules to the first type of complex. In contrast to these results observed for binding to the alternating polynucleotide, only the 1:1 type of complex is formed when this ligand binds to the homopolymer poly(dA) · poly(dT).

【 授权许可】

Unknown