Footprinting studies on the sequence‐selective binding of pentamidine to DNA

FEBS Letters

Stevens, Malcolm F.G.¹  Fox, Keith R.¹  Sansom, Clare E.¹ 

[1] Department Physiology and Pharmacology, University of Southampton, Bassett Crescent East, Southampton SO9 3TU UK

关键词: Pentamidine;    Drug-DNA interaction;    Minor groove binding;   

DOI  :  10.1016/0014-5793(90)81527-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The sequence-selective binding of pentamidine, an antimicrobial aromatic diamidine, has been investigated by footprinting studies on two different DNA fragments using DNase I, micrococcal nuclease and hydroxyl radical as probes. Each probe reveals drug-induced protection from cleavage in AT-rich regions. The best binding sites consist of at least 5 consecutive AT base pairs. Three or less AT pairs do not constitute a pentamidine binding site.

【 授权许可】

Unknown