DNA strand breakage and base modification induced by hydrogen peroxide treatment of human respiratory tract epithelial cells

FEBS Letters

Aruoma, Okezie I.³  Wu, Reen¹  Cross, Carroll E.²  Jenner, Andrew³  Halliwell, Barry³  Spencer, Jeremy P.E.³  Chimel, Ken¹ 

[1] Pulmonary Research Group, California Regional Primate Research Centre, UC Davis, Davis, CA 95616, USA;Division of Pulmonary/Critical Care Medicine, Department of Internal Medicine, UC Davis Medical Centre, Sacramento, CA 95817, USA;Neurodegenerative Disease Research Centre, University of London King's College, Manresa Road, London, SW3 6LX, UK

关键词: Human respiratory tract epithelial cell;    DNA damage;    DNA base modification;    Strand breakage;    H2O2;    GC-MS;    5-OH-uracil;    5-hydroxyuracil;    5-OHMe-uracil;    5-(hydroxymethyl)uracil;    FAPy-adenine;    4;    6-diamino-5-formamidopyrimidine;    8-OH-adenine;    8-hydroxyadenine;    FAPy-guanine;    2;    6-diamino-4hydroxy-5-formamidopyrimidine;    8-OH-guanine;    8-hydroxyguanine;    8-OHdG;    8-hydroxy-2′-deoxyguanosine;   

DOI  :  10.1016/0014-5793(95)01117-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of human respiratory tract epithelial cells with H₂O₂ led to concentration-dependent DNA strand breakage that was highly-correlated with multiple chemical modifications of all four DNA bases, suggesting that damage is due to hydroxyl radical, OH^.. However, the major base damage occurred to adenine. Hence, conclusions made about the occurrence and the extent of oxidative DNA damage on the basis only of changes in 8-hydroxyguanine should be approached with caution.

【 授权许可】

Unknown