Involvement of the three inter‐α‐trypsin inhibitor (ITI) heavy chains in each member of the serum ITI family

FEBS Letters

Héron, Antoine¹  Bourguignon, Jeannette¹  Diarra-Mehrpour, Maryam¹  Sesboüé, Richard¹  Dautréaux, Brigitte¹  Martin, Jean-Pierre¹ 

[1] INSERM Unité 295, Faculté de Médecine-Pharmacie de Rouen, BP 97, Avenue de l'Université, 76803 St. Etienne-Rouvray Cédex, France

关键词: Inter-α-trypsin inhibitor;    Pre-α-trypsin inhibitor;    Recombinant DNA;    Hybrid protein;    Polyclonal antiserum;    Western blotting;    ITI;    inter-α-trypsin inhibitor;    PαI;    pre-α-trypsin inhibitor;    IαLI;    inter-α-like inhibitor;    H1;    H2;    H3 and L;    heavy- and light-chain precursors of ITI-related proteins;    HC1;    HC2 and HC3;    mature heavy chains of ITI-related proteins;    GAG;    glycosaminoglycan;    MBP;    maltose-binding protein;    IPTG;    isopropyl β-d-thiogalactoside;    PAP;    peroxidase antiperoxidase;    DAB;    diaminobenzidine;    EGTA;    ethyleneglycoltetraacetic acid;    SDS-PAGE;    sodium dodecyl sulphate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(95)01103-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Partial cDNAs coding for each of the three human inter-α-trypsin inhibitor (ITI) heavy chains were expressed in a bacterial plasmid system and rabbits were immunised with the fusion peptides obtained. Despite the strong sequence homology of these chains, the antisera turned out to be highly specific in the analysis of corresponding mRNA translation products or partially digested serum ITI. Besides classical serum ITI members, their use in Western blotting made it possible to evidence an H3-related ITI form and a low-amount H1-related HC/bikunin component. The relative levels of ITI family members was further studied in baboon and foetal calf sera.

【 授权许可】

Unknown