Transition metal ions within human atherosclerotic lesions can catalyse the oxidation of low density lipoprotein by macrophages

FEBS Letters

Leake, David S.¹  Lamb, David J.¹  Mitchinson, Malcolm J.² 

[1] School of Animal and Microbial Sciences, University of Reading, Whiteknights, PO Box 228, Reading, Berkshire, RG6 6AJ, UK;Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QP, UK

关键词: Atherosclerosis;    Gruel;    Low density lipoprotein;    Macrophage;    Oxidised low density lipoprotein;    Oxidized low density lipoprotein;    BHT;    butylated hydroxytoluene;    DMEM;    Dulbecco's modified Eagle's medium;    LDL;    low density lipoprotein;    MDA;    malondialdehyde;    TBARS;    thiobarbituric acid-reactive substances;   

DOI  :  10.1016/0014-5793(95)01068-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The oxidation of low density lipoprotein (LDL) in the arterial wall may contribute to atherogenesis. The oxidation of LDL by cells usually requires catalytically active transition metal ions. We show here some that gruel samples from human advanced atherosclerotic lesions are capable of catalysing the oxidation of LDL by macrophages as measured by thiobarbituric acid-reactive substances, enhanced electrophoretic mobility and increased macrophage uptake. This catalysis could be inhibited by pretreatment of the gruel with Chelex-100, which binds transition metal ions. The presence of catalytically active transition metal ions in atherosclerotic lesions may help to explain why LDL oxidation occurs at these sites.

【 授权许可】

Unknown