Detection of β‐1,2‐mannosyltransferase in Candida albicans cells

FEBS Letters

Okawa, Yoshio¹  Oshie, Asayo¹  Kobayashi, Hidemitsu¹  Suzuki, Shigeo¹  Shibata, Nobuyuki¹  Tezuka, Atsuko¹  Suzuki, Akifumi¹  Takata, Yuka¹ 

[1] Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, 4-4-1 Komatsushima, Sendai Aoba-ku, Miyagi 981, Japan

关键词: Candida albicans;    β-1;    2-Linked mannose;    Mannosyltransferase;    Tris;    tris-hydroxymethylaminomethane;    EDTA;    ethylenediaminetetraacetic acid;    PMSF;    phenylmethylsulfonyl fluoride;    HPLC;    high performance liquid chromatography;    PA;    2-aminopyridine;   

DOI  :  10.1016/0014-5793(95)01061-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A particulate insoluble fraction from Candida albicans J-1012 (serotype A) strain cells was obtained as the residue after extracting a 105,000 × g pellet of cell homogenate with 1% Triton X-100. Incubation of this fraction with a mannopentaose, Manβ1 → 2Manα1 → (2Manα1 →)₂2Man (αβMan₅), in the presence of GDP-mannose followed by high performance liquid chromatography showed the formation of a mannohexaose. Analysis of the product by ¹H NMR indicates that αβMan₅ was changed to Manβ1 → 2Manβ1 → 2Manα1 → (2Manα1 →)₂2Man (αβMan₆). This β-1,2-mannosyltransferase (ManTase) II activity was completely inhibited by Zn²⁺ and was not restored by the addition of EDTA. The corresponding enzyme fraction from C. albicans NIH B-792 (serotype B) strain cells, the mannan of which does not possess both the αβMan₅ and αβMan₆ side chains, also exhibited the same β-1,2-ManTase II activity.

【 授权许可】

Unknown