Temperature‐dependent change of aerological specificity of Candida albicans NIH A‐207 cells cultured in yeast extract‐added Sabouraud liquid medium: disappearance of surface antigenic factors 4, 5, and 6 at high temperature

FEBS Letters

Okawa, Yoshio¹  Kobayashi, Hidemitsu¹  Suzuki, Shigeo¹  Miyauchi, Masayoshi¹  Shibata, Nobuyuki¹  Kawamata, Masaki¹  Suzuki, Akifumi¹  Takahata, Takashi¹ 

[1] The Second Department of Hygienic Chemistry, Tohoku College of Pharmacy, 4-4-1 Komatsushima, Sendai Aoba-ku, Miyagi 981, Japan

关键词: Candida albicans;    Cell-wall mannan;    β-1;    2 Linkage;    Phosphodiester;    Antigenic factor;    High temperature;    Aggregation;    YSLM;    yeast extract-added Sabouraud liquid medium;    ELISA;    enzyme-linked immunosorbent assay;    1H NMR;    1H nuclear magnetic resonance;   

DOI  :  10.1016/0014-5793(94)00434-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cells of Candida albicans NIH A-207 strain (A-strain) cultivated in YSLM at high temperatures (37 and 40°C) did not undergo agglutination with the factor sera 4, 5, and 6 in a commercially available factor serum kit, ‘Candida Check’, and formed a grape-like shape. The mannans isolated from the cells had lost their reactivity against the factor sera in ELISA. It was also revealed by ¹H NMR analysis that the mannans contained neither a phosphate group nor a β-1,2-linked mannopyranose unit, although these mannans increased the non-reducing terminal α-1,3-linked mannopyranose unit. The cells and the mannans prepared by cultivation at such high temperatures followed by 27°C in the same medium entirely recovered the reactivity with the factor sera.

【 授权许可】

Unknown