Definitive chemical evidence for the constitutive ability of Candida albicans serotype A strains to synthesize β‐1,2 linked oligomannosides containing up to 14 mannose residues

FEBS Letters

Jouault, T.²  Trinel, P.A.²  Strecker, G.¹  Lepage, G.²  Poulain, D.² 

[1] Laboratoire de Chimie Biologique et Unité Mixte 111, Centre National de la Recherche Scientifique, Université de Lille I, 59655 Villeneuve d'Ascq Cedex, France;Unité 42, Institut National de la Santé et de la Recherche Médicale, Domaine du CERTIA, P.O. Box 39, 59651 Villeneuve d'Ascq Cedex, France

关键词: Candida albicans;    Serotype A;    Mannan;    β-1;    2 linked oligomannoside;    Degree of polymerization;    NMR analysis;   

DOI  :  10.1016/S0014-5793(97)01205-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously reported the presence of phosphate bound β-1,2 linked oligomannosides with unusually high degrees of polymerization (DP>7) in the mannan of Candida albicans strain VW32. To confirm this observation, we have prepared these oligomannosides from the mannan of C. albicans strain NIH A 207. Gel filtration chromatography and TLC analysis revealed DP up to 14. For both strains, NMR analysis confirmed the exclusive presence of β-1,2 linkages in the pools of oligomannosides with a DP higher than 6 which presented an average DP of 10.6 (VW32) and 10.4 (NIH A 207). These results are important to consider in relation with the ability of these C. albicans derived oligomannosides to trigger TNFα synthesis according to their DP.

【 授权许可】

Unknown